Workshop on 3D Spheroid and Organoid Culture-Tools for Human Health Research
23-25 November, 2023
School of Life Sciences and School of Biotechnology, Jawaharlal Nehru University, New Delhi, India

8th Hands-on Workshop for Handling and Care of Laboratory Animals
28 November - 2 December 2023
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

Topics

Fundamentals of stem cells/spheroids/organoids
CSCs niches and tumor microenvironment
Multicelluler/single-cell derived spheroids
Techniques of spheroid/organoid culture
Requirements for organoid/spheroid culture
Scaffold-free spheroid culture
Ultra-low attachment culture
Hanging drop method
Scaffold-based spheroid culture
Matrix-on-top and matrix-embedded methods
Agarose-based method
Microfluidics device-based method
Microgravity-based spheroid formation
Characterization of spheroids/organoids
Growth kinetics of spheroids/organoids
Morphological features
Role of spheroid/organoid culture in predicting drug sensitivity/ resistance, tumor heterogeneity, and tumor evolution

Brochure Details

Topics

Introduction to animal world - Laboratory animals
Biology of mice, rat and rabbit - Anatomy
Ethical Committee - Ethics for using animals in research
Administrative requirements for animal experiments
Perioperative care, anesthesia, surgical techniques and analgesia
Pain: causes, category monitoring and distress in laboratory animals
Euthanasia in laboratory animals
Disease-specific animal models - Cancer
Animal models in immunology research - Alternatives
Learning and memory in animal model
Power of statistics in data analysis for animal experiments
In vivo animal imaging system
Genetics of laboratory animals - transgenics and knockouts
Preparation of experimental protocols; critical analysis of scientific papers with animal experiments